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Differential Expressions of Porcine IGF-1 E Peptides in Pigs of Different Body Sizes |
LI Changhong1, LI Nannan2, FENG Wanjuan1, CHENG Hua1, HAO Linlin2, WEI Wenzhen2** |
1.College of Life Science, Baicheng Normal University, Baicheng 137000, China; 2.College of Animal Science, Jilin University, Changchun 130062, China |
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Abstract IGF-1 gene is thought to be related to the “proportional dwarfism” of minipigs, the structure of which is complex and the Cterminal of which can produce three types of E-peptides (Ea, Eb and Ec) due to variable splicing. In this study, Large White and Bama pigs were chosen as representatives of large and small pigs. Identification and relative expression were detected in large and small pigs by PCR and real-time fluorescent quantitative PCR. The results showed that there were 3 types of E peptides in large and small pigs, among which Ea was highly expressed in minipigs (P<0.05), especially in liver, heart,lung and spleen tissues; Eb was in the liver of minipigs. The expression level in cartilage tissue was higher, but in heart issue, the expression level was higher in large pigs, while the expression level in other tissues was lower; Ec was similar to Ea in large and small pigs. Compared with the three E peptides, the expression level of Ec was the highest, the expression level of Ea was the second, and the expression level of Eb was the lowest. The results showed that the high expression of Ea and Ec was not conducive to the growth of pig body length, which provides a more comprehensive theoretical basis for the analysis of the application of small pigs and small pigs.
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Received: 15 October 2019
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