Abstract In this study, electroantennogram (EAG) and Y tube olfactomcter were used to screen the attractive and repellent compounds from 8 kinds of volatiles from soybean leaves on Colposcelis signata adults to provide theoretical basis for exploring biological agents to prevent this pest. EAG bioassay results showed that the relative values of EAG response increased with the increase of volatile concentration and the female was stronger than the male. At the same concentration, the relative value of EAG response for 1-octene-3-ol was the largest, followed by 3-octanol and (Z)-3-hexene-1-ol. As for behavioral response, there were differences among lure rate or repellent rate of different volatiles at the same concentration. 1-octen-3-alcohol, 3-octanol, (Z)-3-hexen-1-ol, 3-octanone, methyl jasmonate and N, N-dimethyl cacetamide had significant attractive effects. With the increase of concentration,lure rate increased. 1-octene-3-ol at 10-1 mol/L was the most attractive to females and males, which were 90.00% and 84.45%, respectively. Repellent rate of hexanal was the highest, which was significantly higher than that of other plant volatiles. β-caryophyllene at low concentration of 10-5-10-4mol/L could attract females and males, but at high concentration of 10-2 -10-1 mol/L, it had significant repellent effect.
|