Comparative Analysis of Non-targeted Metabolomics between Rump and Eye Tissues of Yanbian Yellow Cattle
SUN Bin1，CUI Yan1，WANG Weili2，TANG Lin1，WANG Ying1，LI Qiang1，LI Xiangzi1**
1. Northeast Cold Region Beef Cattle Technology Innovation Engineering Research Center of the Minis?
try of Education/Jilin Province Beef Cattle Science and Industrial Technology Major Demand Collab?
orative Innovation Center，Yanbian University，Yanji 133002，China；2. Changchun Customs Technol?
ogy Center，Changchun 130062，China
Abstract：The experiment aims to analyze the differences in metabolite profiles between rump and
eye tissues of Yanbian yellow cattle from non-targeted metabolomics.The experiment used the rump
and eye tissues of 6 Yanbian yellow cattle of the same month age and growth conditions as experimen?
tal materials. The metabolites were separated by GC-MS, and the data were analyzed by statistical
methods such as PCA, OPLS-DA and UVA.The results were as follows: The experiment screened out
differential metabolic pathways,including phenylalanine metabolism,glyoxylic and dicarboxylic acid metabolism, pentose phosphate pathway, glycine,serine and threonine metabolism,arginine and pro?
line metabolism, and purine metabolism. The results of the screening of differential metabolites in
rump group and eye group were as follows: 20 different metabolites were screened out, of which 11
were upregulated (P<0.05),and 9 were downregulated (P<0.05). In summary, non-targeted metabolo?
mics analysis reveals significant differences in metabolic levels between rump meat and eye meat.
Different metabolites such as oleic acid will serve as precursors of flavor compounds and thus affect
flavor.
