Abstract:In order to investigate the effects of exogenous glutathione (GSH) on the growth and physiology of cucumber seedlings under soil atrazine stress, this experiment set up exogenous GSH treatment with different concentrations (0, 50, 100,200 mg/L), simulated the residual concentration of atrazine in soil (0.20 mg/kg), and conducted cucumber seedling experiments. The results showed that under soil atrazine stress, 100,200 mg/L exogenous GSH treatment had a significant effect on the fresh weight of cucumber seedlings. The leaf area of seedlings treated with 100 mg/L was significantly increased compared with the control. At the same time, 100,200 mg/L exogenous GSH significantly increased sod superoxide dismutase (SOD) and peroxidase (POD) enzyme activities, chlorophyll content, leaf net photosynthetic rate and GSH content, glutathione reductase (GR) activity, and glutathione-s-transferase (GSTs) activity, while MDA content significantly decreased. Among them,100 mg/L exogenous GSH treatment had the most significant effect on seedlings, which reduced the toxicity of atrazine to cucumber seedlings.