Abstract Through a variety of methods, such as bacterial morphology,physiological and biochemi?
cal characteristics and 16S rRNA sequencing, the strain isolated from layer feces was identified as
Bacillus licheniformis.The probiotic effect of Bacillus licheniformis LH96 was evaluated by the in vi?
tro method, and it was found that it reached a stable period at 14 h, and it had good heat resistance. It
had good tolerance under the conditions of pH 2.5-5.0,0.1%-0.3% bile salt concentration and simu?
lated artificial gastrointestinal fluid. B. licheniformis LH96 had the ability to produce a variety of
short-chain fatty acids, of which the ability to produce acetic acid was the strongest.The results of the
antibiotic resistance test showed that the strain was resistant to azithromycin, clindamycin and eryth?
romycin.At the same time,the whole genome information of B.licheniformis LH96 was obtained by
gene assembly.The results showed that the strain was 4 187 148 bp, with a G+C content of 46.18%
and a total of 4 263 protein coding sequences. The strain contains a large number of genes related to
carbohydrate metabolism, as well as glycoside hydrolases, polysaccharide lyases and carbohydrate es?
terases. The enzymes in these metabolic pathways can help break down nutrients such as starch, cel?
lulose and pectin that chickens cannot digest.The results provide a genetic basis for further elucidat?
ing the functional mechanism of its probiotic properties.
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Received: 09 January 2021
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