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| Traits of Muscle Fiber in Different Parts of Berkshire×Landrace× Yorkshire Porcine Hybrid Pigs |
| GAO Yi1，LIU Hongliang1，YU Yongsheng2，LI Zhaohua1，JIN Xin1，ZHANG Guoliang1**，LI Na1** |
| 1. Institute of Animal Science and Veterinary Medicine，Jilin Academy of Agricultural Sciences，Gong?
zhuling 136100，China；2. Institute of Animal Biotechnology，Jilin Academy of Agricultural Sci?
ences，Gongzhuling 136100，China |
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Abstract In order to explore the muscle fiber traits of different parts of pigs, four tissues of longissi?
mus dorsi, psoas major, subscapularis and adductor of berkshire×landrace×yorkshire (BLY) hybrid
pigs were studied. The diameter, cross-sectional area and density of muscle fibers in different parts
were observed by HE staining, and the expression percentage of fast and slow muscle fibers in differ?
ent parts was detected by immunohistochemistry. Finally, qPCR was used to analyze the expression
of MyHCⅠ/MYH7 (slow oxidizing type), IIa/MYH2 (fast oxidizing type), IIx/MYH1 (oxidation and gly?
colysis type) and IIb/MYH4 (fast glycolysis type) genes in the muscles of different parts. The results
showed that fast muscle fibers accounted for 76%-91% in longissimus dorsi, psoas major, adductor
and subscapularis muscles, and slow muscle fibers accounted for 9%-23%. Subscapularis muscle
had the best meat quality with the highest muscle fiber density and the highest content of oxidized
muscle fibers. The muscle fiber density of psoas major was the lowest and the lowest content of oxi?
dized muscle fibers was longissimus dorsi muscle. The study aims to collect data for the improvement
of pig breeding and muscle quality by analyzing the muscle fiber traits in different parts of pigs.
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Received: 16 August 2021
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