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| Effects of Pretreatment and Ligninase on Enzymatic Hydrolysis and Saccharification of Corn Stalk |
| ZHAO Wenxuan**，JIAO Hongguan，ZHENG Yaqiang |
| College of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China |
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Abstract Corn stalk was selected as the research material, enzymatic hydrolysis and saccharifica?
tion analysis, Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy were used
as detection indexes, and the high-efficiency pretreatment method and conditions of corn straw were
established. The synergistic effects of ligninase, cellulase and xylanase in enzymatic hydrolysis and
saccharification were studied, and the optimal addition ratio and amount of lignocellulase in the pro?
cess of enzymatic hydrolysis and saccharification were also discussed. The results showed that 2%
CaCO3+ 1% H2O2 could remove lignin and retain cellulose and hemicelluloses well at ratio of 1∶11,
temperature of 120 ℃, and time of 70 min. Enzymatic hydrolysis saccharification experiment showed
that laccase in ligninase played a major role in promoting the hydrolysis of pretreated corn straw, and
the optimal addition amount of each ligninase was laccase 8 U/g, lignin peroxidase 10 U/g and man?
ganese peroxidase 6 U/g, respectively. Under these conditions, the mixed sugar content in the hydro?
lyzate was 116 mmol/L, which was 16% higher than that without ligninase added. This study lays
foundation for the establishment of a high-efficiency lignocellulose-degrading complex enzyme sys?
tem with low enzyme dosage and high sugar yield and the efficient conversion and utilization of corn
stalk lignocellulose raw materials.
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Received: 29 September 2020
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