In order to develop a detection method for diagnosing Brucella in velvet antler and deer blood rapidly, and to ensure the safety of related deer products used for food and medicine, primers and probes were designed and synthesized on the base of the specific gene IS711 of Brucella. A realtime fluorescence quantitative PCR method was established. The reaction conditions were optimized and a standard kinetic curve, Y=-3.14X+37.62，R2=0.997,was drawn. The method has good reproducibility, sensitivity and specificity. The standard deviation of Ct value for intragroup and intergroup reproducibility tests is less than 0.5, the variation coefficients are all less than 2%, and the minimum detectable copy number is 2.65×101Copies/μL, with good specificity for Brucella abortus IS711 gene. The results showed that the method is sensitive to target gene detection and can be used for the detection of fresh velvet antler, deer blood and other related samples. The method can also be used for the qualitative and quantitative detection of Brucella, providing an important technical guarantee for the quality and safety assessment of related deer products. 