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Abstract The purpose of this study is to analyze and investigate stress resistance and growth characteristics of Lactobacillus amylovorus L-102 and Lactobacillus johnsonii L-76 from swine, and to provide a theoretical basis for further in vitro studies of lactic acid bacteria. Acidbase tolerance, bile tolerance, trypsin tolerance, adhesiveness, bacteriostatic ability, acid production ability, growth curve and pathogenicity of Lactobacillus L-102 and L-76 were measured and analyzed. The results showed that Lactobacillus L-102 and L-76 exhibited resistance to acidbase environment with pH values of 2-11, and could tolerate 0.1%-0.2% bile salts and 1% trypsin in growth medium. The two Lactobacillus strains had certain adhesion to IPEC-J2 cell and inhibited the growth of Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus. Moreover, the range of acid production of Lactobacillus L-102 and L-76 is 4.10-4.30. After 4 hours of culture, it entered the logarithmic phase of growth. The viable count achieved 107 CFU/mL when Lactobacillus L-102 and L-76 were sustained at 50 ℃ and 60 ℃ for 5-15 min. No death mice were found in mice injected intraperitoneally and administered intragastrically with Lactobacillus L-102 and L-76. Data indicated that Lactobacillus amylovorus L-102 and Lactobacillus johnsonii L-76 have potential probiotic characteristics and could develop into feeding probiotic lactic acid bacteria for swine industry.
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