Pluronic is a classical thermosensitive polymer that achieves solgel transition with the change of temperature.However, the structure of hydrogel is unstable, and it is difficult to preserve long, and has a single function in wound dressing application. In this research, the thermosensitive composite hydrogel was synthesized by mixing pluronic F127 and F68 with an antibacterial component CS which can also be used for irregular wound healing. The temperature sensitivity, microscopic morphology, antibacterial activity and the fit to the wound were investigated by test tube inversion, SEM, bacterial coating method and wound model. The results showed that the composite hydrogel was thermosensitive, and formed gelatin at body temperature. The F127/F68/CS hydrogel showed a structure feature of high porosity and 3dimensional networks. The antibacterial was gradually increased with the increase of CS. And the hydrogel could adhere tightly on the wound. F127/F68/CS composite hydrogel is expected to reduce wound infection and promote wound healing due to its convenience of preparation, temperature sensitivity, suitable porous structure, antibacterial properties and good wound adhesion. 