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| Mechanism of the Effects of Molting Extracts on Hela Cell Proliferation and Apoptosis |
| CHEN Yanyan1, YU Bo1, WANG Siming1，ZHANG Wanying1, LI Xiugang2, LI Yinqing1** |
| 1. Jilin Ginseng Academy,Changchun University of Chinese Medicine，Changchun 130117，China; 2. Jilin Province Zhengkai Pharmaceutical Chain Co., Ltd., Changchun 130117，China |
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Abstract By detecting the effects of Ailanthus altissim bark extract on the viability of cervical cancer Hela cells, and observing the regulatory effects of drugs on apoptosis, cell cycle, autophagy and related factors, we preliminarily studied the molecular mechanism of cell apoptosis. Hela cells were cultured in vitro, and cervical cancer Hela cells were treated with different mass concentrations of Ailanthus altissima bark extract. MTT method was used to detect the effect of drugs on the proliferation of Hela cells; Annexin V-FITC/PI double staining flow cytometry was used to detect the effect of Ailanthus altissima bark extract on Hela cell apoptosis; PI single staining flow cytometry was used to detect the effect of Ailanthus altissima bark extract on the cell cycle of Hela; qRT-PCR was used to detect the effect of Ailanthus altissima bark extract on the expression of Bcl-2/Bax mRNA; Western-blot method was used to determine the expression of Hela cell apoptosis factors Bcl-2, Bax and autophagyrelated factor proteins. MTT results showed that Ailanthus altissima bark extract could inhibit the proliferation of cervical cancer Hela cells in a dosedependent manner (P<0.05); After treating Hela cells with the mass concentration of 5, 10, and 20 μg/mL Ailanthus altissima bark extract for 48 hours, the proliferation inhibition rate and apoptosis rate of Hela cells increased significantly, and were higher than those of the control group (P<0.05); The results of PI single staining flow cytometry showed that Ailanthus altissima bark extract induced block Hela cell division in G2/M phase (P<0.05); The results of qRT-PCR showed that Bax mRNA expression increased, and Bcl-2 mRNA expression decreased in a dosedependent manner (P<0.05); Western-blot results showed that Bax protein expression increased, Bcl-2 protein expression decreased, and LC3BⅡ/Ⅰ value increased in a dosedependent manner (P<0.05). Ailanthus altissima bark extract can significantly inhibit the proliferation of cervical cancer Hela cells, by regulating the expression of Bax and Bcl-2 apoptotic genes, blocking the G2/M phase of cells, promoting cell apoptosis, and promoting autophagy in cervical cancer cells by regulating the expression of LC3B gene.
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Received: 29 May 2019
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