This study isolated and purified two strains JL-006 and JL-007 firstly from naturally entomopathogeninfected Myzus persicae and Trialeurodes vaporariorum, which were identified to be Lecanicillium longisporum and Lecanicillium lecanii by morphology and rDNA-ITS sequence analysis. To assess the biological control potential, the pathogenicity of JL-006 and JL-007 with another two fungal strains Lecanicillium attenuatum JL-003 and Beauveria bassiana JL-005 to 3rdinstar nymphs of M. persicae and T. vaporariorum were determined using the dipping method. The results indicated that the virulence was higher at the concentration of 1.0×108 conidia/mL than at 1.0×1.5 conidia/mL. The strains B. bassiana JL-005 and L. longisporum JL-006 were promising candidates for biological control of M. persicae with the higher mortalities of 71.8% and 74.5%, and the lower LT50 values of 5.6 d and 5.5 d, respectively, after inoculation for 10 days. Meanwhile, the strains B. bassiana JL-005 and L. lecanii JL-007 were promising candidates for biological control of T. vaporariorum with the higher mortalities of 82.3% and 80.5%, and lower LT50 values of 5.4 d and 5.1 d, respectively. Our results provide a technical support for simultaneously suppressing several insect pests by a pathogenic fungus in greenhouse. 