In order to optimize the genetic transformation receptor system of soybean, the affecting factors of seed germination and adventitious bud induction were investigated by orthogonal test design on soybean varieties JN18, JN28 and JN54. The results showed that the optimal condition for soybean seed germination is to select JN28 soybean sterilized by sodium hypochlorite solution for 10 min, and inoculated on MS+0.2 mg/L 6-BA germination medium (pH 6). The optimal condition for adventitious bud induction of soybean cotyledon node is to inoculate the seeds germinated for 5 d on MS+3.0 mg/L 6-BA + 0.2 mg/L IBA medium. According to the obtained optimal conditions, the Agrobacteriummediated transformation for JN28 was applied, and the positive transformation rate of PCR was 5.56%. This result lays foundation for the identification of soybean gene function and the acquisition of new genetically modified soybean lines with independent intellectual property rights. 