Abstract：Twelve components including eucommia ulmoides powder and rumen bypass glucose
were mixed in a certain proportion. Six healthy cows and thirty cows with clinical ketosis were se‐
lected for toxicity experiment of energy supplement and therapeutic effect evaluation of ketosis in
cows.The results showed that continuous administration of energy supplement for 7 days in healthy
perinatal period did not induce the damage of liver and kidney function.After continuous administra‐
tion of energy supplement for 3 days in cows with ketosis,the concentration of β-hydroxybutyric acid
and free fatty acid in blood was significantly decreased,while the concentration of glucose was signifi‐
cantly increased.The results of oxidative stress related indicators showed that administration of en‐
ergy supplement markedly increased the activities of peroxidase marker catalase(CAT) and glutathi‐
one peroxidase(GSH-PX) and decreased the malondialdehyde(MDA). The blood concentrations of in‐
flammatory cytokines IL-6, IL-8, and TNF-α were significantly decreased. In addition, it can im‐prove the liver injury as evidenced by decreased activity of alanine aminotransferase(ALT), aspartate
aminotransferase(AST),total bilirubin(TBIL) and indirect bilirubin (IBIL). Moreover, the phosphoryla‐
tion of hepatic energy metabolism and pathological injury regulator AMPK and SIRT3 protein con‐
tent were significantly increased. These results indicate that energy supplement administered by ga‐
vage can effectively relieve the negative energy balance, oxidative stress,inflammatory reaction, and
liver injury of ketosis in dairy cows.
