1. College of Chinese Medicinal Materials, Jilin Agricultural University, Laboratory for Cultivation
and Breeding of Medicinal Plants of National Administration of Traditional Chinese Medicine,Chang?
chun 130118, China;2. Jilin Province Engineering Research Center for Ginseng Genetic Resources
Development and Utilization, Changchun 130118, China;3. Ginseng and Antler Products Testing
Center of the Ministry of Agriculture and Rural Affairs of the People's Republic of China,Changchun
130118,China
Abstract:In order to enrich ginseng growth promoting rhizobacteria, this study used ginseng rhizosphere soil as materials to isolate and screen plant growth promoting rhizobacteria with probiotic potential by using functional medium, and isolated strains were classified and identified by morphology,physiology, biochemistry and 16S rDNA sequencing, and the effects of the screened PGPR on thegrowth of ginseng seedlings were verified by pot test. The results showed that 17 strains of PGPR were isolated, including 10 strains with phosphorus solubilizing ability, 8 strains with iron carrier producing ability, 7 strains with IAA producing ability, 9 strains with nitrogen fixing effect, and 10 strains with two or more different promoting functions. They are from 8 different genera, Pseudomonas, Burkholderia, Bacillus, Enterobacter, Agrobacterium, Paenarthrobacter, Acinetobacter, Leclercia and Priestia, among which the largest number of Pseudomonas accounted for 29.41%. Strain F05-44 had the strongest overall growth-promoting ability, and the pot test found that this strain promoted the growth of ginseng without fertilization, and plant height and root length increased by 38.53% and 24.65%, respectively, and leaf spreading period was shortened by 3-4 days, which has potential application value.