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Journal of Jilin Agricultural University
2020, Vol.42 Num.5
Online: 2020-11-13


 
473 DOU Sen
Protection and High-value Utilization Engineering of Mollisol Land in Jilin Province Hot!
 Mollisol land in Jilin province refers to the highquality land with black or dark humus topsoil, good properties and high fertility in the administrative region of the province, specifically including black soil, chernozeme, meadow soil, dark brown soil, brown soil, Baijiang soil and paddy soil. According to this definition, through the superposition analysis of soil types and land type data, the total black land area of Jilin province is 154 600 km2, accounting for 83.3% of the land area of Jilin province. Although Jilin province takes the lead in the protection of mollisol land in China, the problems of “mollisol land becoming less”“mollisol land layer becoming thinner” and “mollisol land becoming infertile” have not been completely controlled. The highvalue utilization mode and brand that use mollisol land to produce highvalue green food and transform resource advantage into economic advantage have not yet formed. It is urgent to carry out research on mollisol land protection and highvalue utilization from the perspective of engineering implementation. It is suggested to carry out engineering projects such as topsoil stripping and utilization of occupied cultivated land by construction projects, construction of highstandard farmland, land fertility improvement of cultivated land, and soil improvement of obstructed cultivated land.
2020 Vol. 42 (5): 473-476 [Abstract] ( 29 ) [HTML 1KB] [PDF 1600KB] ( 29 )
477 CHE Dongsheng, LI Huijuan, ZHAO Bao
Progress in the Study of Metabolic Mechanism of Homoarginine Hot!

Homoarginine (hArg) is an endogenous nonprotein amino acid, a pair of homologous amino acids to Arg, and is the synthetic substrate of NO synthesis. Recent studies have shown that hArg plays an important role in cardiovascular, liver, neurological function, and intrauterine growth restriction. However, compared with Arg, the application of hArg in human and animal research is still at an early stage, and its mechanism of action has not been fully proved. Therefore, the synthesis, transport and metabolic pathways of hArg were reviewed to provide theoretical support for the application of functional amino acids in animal production.

2020 Vol. 42 (5): 477-485 [Abstract] ( 23 ) [HTML 1KB] [PDF 1469KB] ( 5 )
486
Yield and Photosynthetic Characteristics of Different Paternal Blood Maize Hybrids

In order to reveal the changes in yield, dry matter accumulation, transport and photosynthetic characteristics of different paternal maize hybrids,the hybrids of Tangsipingtou bloodline, Luda red bloodline, Lancaster bloodline and tropical bloodline hybrids were used as experimental materials, and their yield, dry matter accumulation, transport and photosynthetic characteristics were analyzed by routine statistical analysis. The results showed that performances of both yield and net photosynthetic rate were as follows: hybrids with Tangsipingtou bloodline as male parent>hybrids with tropical bloodline as male parent>hybrids with Lancaster bloodline as male parent>hybrids with Buda red bone bloodline as male parent. The hybrids with Tangsipingtou bloodline as male parent had the highest contribution to dry matter accumulation, dry matter transfer and transfer rate, and dry matter transfer to grain. Correlation analysis showed that there was a significant positive correlation between yield and net photosynthetic rate and dry matter transfer rate (R=0.982*, 0.956*). In this experiment, the hybrid combination with Tangsipingtou bloodline inbred line as male parent and Reid bloodline inbred line as female parent was superior to other combinations in yield, dry matter accumulation and photosynthetic characteristics, which could increase the success rate of breeding.

2020 Vol. 42 (5): 486-492 [Abstract] ( 25 ) [HTML 1KB] [PDF 1067KB] ( 4 )
493 LIU Yueyue, HUANG Xiaoxuan, GENG Yanqiu, GUO Liying, JIN Feng**, SHAO Xiwen**
Effects of Soda Saline-alkali Stress on Growth and Nitrogen Metabolism Key Enzyme of Rice
In order to clarify the differences in growth and nitrogen metabolism of rice seedlings of different ages under soda salinealkaline stress, Changbai 9 was used as test material, rice seedlings of different ages, L1 (3-leaf seedling age), L2 (4-leaf seedling age) and L3 (5-leaf seedling age), were stressed with 50 mmol/L mixed saline alkali, and the physiological response, material accumulation and nitrogen absorption characteristics of the rice seedlings were studied after soda salinealkali stress (0-48 h). The results showed that soda salinealkali stress significantly increased the content of proline and soluble sugar in rice seedlings, the response time of the 3-leaf seedlings was earlier and the increase was greater, and with the increase of age, the osmotic adjustment ability became weaker; Soda saline-alkali stress significantly increased Na+ accumulation and Na+/K+ in leaves and roots of rice seedlings of all ages, the peak value of 3-leaf seedlings was higher, and the ion balance was gradually enhanced with the growth of rice seedlings. From the perspective of nitrogen metabolism, soda salinealkali stress significantly inhibited nitrogen uptake and transformation in different seedling ages, the activities of NR and GS in leaves and roots were reduced, and nitrogen accumulation in shoots and dry matter accumulation in plants were inhibited. The decrease of NR and GS activities in the 5-leaf seedlings was significantly less than that of other seedlings, and the GDH metabolic pathway was enhanced by slightly upregulating the GDH activity of leaves, so as to adapt to the effects of salinealkali stress on nitrogen uptake. The effects of 5leaf seedlings on shoot dry weight, root dry weight and nitrogen accumulation were also significantly less than those of other seedling ages. The above results show that transplanting rice in soda salinealkaline land to select ageing seedlings with large leaves is beneficial to enhance seedling resistance and ensure more efficient nitrogen absorption and material accumulation.
2020 Vol. 42 (5): 493-501 [Abstract] ( 16 ) [HTML 1KB] [PDF 1054KB] ( 5 )
502 MENG Xiangpeng, SONG Yang, JIN Yukun, ZHANG Lin, WANG Piwu
Identification of Soybean Lines with hrpZPsta Gene Resistant to Cercosporidium sofinum

T5/ T6 generation of JNhrpZPsta2004-30-384 transgenic plants were used as the source of plant materials. The molecular detection and stability identification of T5 and T6 soybean lines were carried out by PCR, Southern blot and qRT-PCR techniques, and then indoor artificial inoculation was used for disease resistance analysis. The results show that the CaMV35S promoter, Nos polyA terminator, target gene and Bar gene were expressed in transformed soybean, indicating that the target gene was stably inherited. The Southern blot assay revealed that multiplecopy of the fragment was inserted in the transformed soybean, and the site was different. The qRT-PCR detection results indicate that the target gene was expressed in all organs (leaves, shoots and roots) of the transgenic soybeans, with the highest expression level in leaves and the lowest in roots. The hrpZPsta expression of roots, shoots and leaves in T5 generation was 2.871, 1.363 and 7.743, respectively, and the hrpZPsta expression of roots, shoots and leaves in T6 generation was 3.577, 1.664 and 8.589, respectively. In accordance with the “identification of soybean gray spot disease technical specifications”, the results suggest that the disease index of T5/T6 untransformed soybean was 52.4% and 50.6%, respectively, and the disease index of T5/T6 transformed soybean was 38.7% and 38.4%, respectively. The untransformed soybean belonged to the medium susceptibility level and transgenic soybean belonged to the medium resistance level. 

2020 Vol. 42 (5): 502-509 [Abstract] ( 18 ) [HTML 1KB] [PDF 4290KB] ( 2 )
510 ZHANG Jian, CHI Yujie, ZHAO Qingquan, FENG Lianrong
Expression of Cytochrome P450 Genes from Lenzites gibbosa Treated with Alizarin Red
The cultures of white rot fungus Lenzites gibbosa were treated with anthraquinone dye alizarin red for 0 h (ck), 3 h (QSH1), 7 h (QSH2), and 10 h (QSH3), respectively, and 4 transcriptomes were constructed so as to provide support for the study on the role of cytochrome P450 (CYP450) genes in the decolorization and degradation of alizarin red by white rot fungi. The highthroughput sequencing technology was used to perform transcriptome sequencing on the hyphal samples under the treatment of alizarin red. The cytochrome P450 genes were screened out using functional annotation. Results showed that a total of 26.17 Gb of valid data were obtained from the four transcriptomes, and the mapped ratios between the reads and reference genome of each sample ranged from 71.23% to 73.66%. Ninetysix CYP450 genes were gained through searching all genes by functional annotation. Differential expression screening was performed for all CYP450 genes by comparing ck with QSH1, ck with QSH2, and ck with QSH3. Three of the genes, gene_24255, gene_18992, and gene_7490,  were found in all three comparative groups. The expression levels of the three genes at 0-10 h were compared with the decolorization rate of alizarin red, and the expression quantities of the three genes were positively correlated with decoloring rate. Through the analysis of the KEGG pathway, gene_25488 was annotated into the pathway of aromatic degradation.The results indicated that CYP450 genes may be involved in the decolorization of alizarin red dye by L.gibbosa. This study, at the transcriptional level, preliminarily analyzed the CYP450 genes and their products of L. gibbosa, which may be involved in the related metabolic pathways and biological processes of degradation and decolorization of alizarin red. The results obtained have important reference value for the subsequent research on the degradation mechanism of alizarin red.
2020 Vol. 42 (5): 510-517 [Abstract] ( 21 ) [HTML 1KB] [PDF 2643KB] ( 2 )
518 CHEN Yue, LI Hulin, YAN Han, LANG Bin, ZHU Shimiao, JI Wenxiu**
Isolation of Antagonistic Bacteria against Tobacco Anthracnose and Optimization of Fermentation Conditions
In order to obtain the antagonistic bacteria and the optimum fermentation conditions of tobacco anthracnose, the bacteria in the rhizospheric soil, roots, stems, and leaves of tobacco were isolated. Accordingly, the antagonistic bacteria of tobacco anthracnose were screened by the method of solid and liquid medium and their antagonistic activity was determined, as well as identified by 16S rRNA analysis. The single factor experiment of shake flask fermentation and the response surface method based on Box-Behnken design were used to optimize the fermentation conditions of the best antagonistic bacteria. The results showed that there were 31 strains with antagonistic effects against tobacco anthracnose, and 10 strains with inhibition rate greater than 30%. The strain with the highest inhibition rate was C-4, and the inhibition rate was 72.14%. Sequence analysis initially identified it belonged to the genus Bacillus. The optimal fermentation conditions for Bacillus C-4 were as follows: inoculum volume 4%, initial pH 7.1, culture temperature 25.5 ℃, liquid volume 84.5 mL, shaker speed 180 r/min, and culture time 48 h. It was verified that under the optimal conditions, the OD600 value of the fermentation broth was 1.859, which was 1.62 times of that before optimization, and the inhibition rate was increased by 8.4%. This study provides an available strain resource for the development of microbial inoculant for the control of tobacco anthracnose. It is effective to optimize fermentation conditions of antagonistic bacteria of tobacco by using the response surface method.
2020 Vol. 42 (5): 518-525 [Abstract] ( 17 ) [HTML 1KB] [PDF 1707KB] ( 3 )
526 ZHANG Jiejing, CHEN Deguo, GAO Jixuan, ZHANG Jianfeng, TIAN Chunjie, TIAN Lei
Screening and Identification of an Antagonistic Bacteria against Ginseng Cylindrocarpon Destructans and Its Effect on Microbial Diversity in Ginseng Rhizosphere Soil Hot!

 The pesticide residues of ginseng will exceed the standard and affect the medicinal value of ginseng.It is one of the potential biological control measures to use biocontrol bacteria to control ginseng diseases.A biocontrol bacterium C10 was isolated from ginseng rhizosphere soil by soil dilution method.It was identified as Bacillus amyloliquefaciens. The results showed that the antibacterial rate of the strain was 80.95%. The results showed that Bacillus amyloliquefaciens C10 could significantly reduce the infection of rust rot fungi on ginseng stems and roots. In addition, microbial diversity analysis showed that inoculation of Bacillus amyloliquefaciens C10 significantly reduced the richness index of fungi in ginseng rhizosphere soil. It can be seen that the use of this fungus has a positive effect on the prevention and control of ginseng rust rot.

2020 Vol. 42 (5): 526-532 [Abstract] ( 18 ) [HTML 1KB] [PDF 1553KB] ( 3 )
533 TONG Rong, QIAO Hongyu, WANG Zhibo, WU Chunyan, CHEN Shanshan, SUN Yuqing, YU Zhandong
Construction of Bivalent Antisense Vector and Its Genetic Transformation to Chinese Cabbage

In order to obtain the cabbage plant resistant to turnip mosaic virus disease and bolting, the construction and genetic transformation of bivalent antisense plant expression vectors were studied. Using TuMV-Nib and LFY genes, an antisense bivalent plant expression vector was constructed and transformed into Agrobacterium LBA4404. Based on the engineering strain, Agrobacteriummediated vacuum infiltration method was used to transform 4 Chinese cabbage inbred lines, and 16 transgenic plants identified by PCR and TuMV infection were obtained. Seven strains were identified as resistant to TuMV virus and bolting in the field. It provides germplasm materials for transgenic Chinese cabbage resistant to antiviral diseases and bolting.

2020 Vol. 42 (5): 533-537 [Abstract] ( 22 ) [HTML 1KB] [PDF 2670KB] ( 2 )
538 WANG Ting, WANG Yujun, ZHANG Xiufang, MA Xiulan, GAO Hongjun
Adsorption Characteristics and Influencing Factors of Exogenous Nitrogen in Acidic Protected Black Soil
To clarify the migration activity of exogenous nitrogen on acidified black soil in protected area, batch equilibrium adsorption experiment was used to explore the adsorption characteristics of nitrogen under different influencing factors. The results showed that the adsorption process of nitrogen in acidified black soil in protected area included rapid, decelerated and equilibrium stages, quasisecondorder kinetics combined with Elovich equation could describe the adsorption kinetics of nitrogen in acidified soil in protected areas; With the increase of nitrogen concentration, the adsorption capacity of the tested soil to nitrogen increased first and then tended to balance, and the adsorption capacity to nitrogen increased with the increase of ambient temperature. When the content of organic matter in soil decreased from 33.95 g/kg to 19.9 g/kg, the saturated adsorption capacity decreased by 17.4%. The addition of exogenous ions in the background solution inhibited the adsorption of nitrogen by soil. The alkalization of the tested soil could promote nitrogen fixation and reduce nitrogen loss. The results could reveal the environmental behavior of nitrogen in acid soil and provide theoretical basis for reducing groundwater pollution.
2020 Vol. 42 (5): 538-544 [Abstract] ( 21 ) [HTML 1KB] [PDF 0KB] ( 1 )
545 HUANG Ying, DOU Sen,GAO Hongjun,DONG Weihua
Effects of Corn Strover Deep Incorporation with Different Amounts on the Composition of Humus in Black Soil Hot!

In this study, the effects of corn stover deep incorporation with different amounts on the composition of black soil humus were studied for solving the problems of stover utilization and soil subsurface fertilization. By taking black soil in the Teaching and Scientific Research Base of Jilin Agricultural University as research object, 5 treatments of ck, 1/2CS, CS, 2CS and 3CS were designed, which respectively represent no corn stover application, halfamount corn stover deep incorporation (4.5 t/hm2), fullamount corn stover deep incorporation (9.0 t/hm2), double of corn stover deep incorporation (18.0 t/hm2) and triple of corn stover deep incorporation (27.0 t/hm2), and then water soluble substance, humic acid, fulvic acid and humin were extracted by humus composition modification method. The results showed that corn stover deep incorporation could effectively increase soil fertility and improve humus composition, and molecular structure of HA and FA developed in the direction of simplification, among which the effect of fullamount corn stover deep incorporation (CS) was the most obvious. Compared with ck, full-amount corn stover deep incorporation (CS) also significantly increased the contents of soil available nutrients (N, P and K), among which the growth of alkalihydrolyzable nitrogen content in surface and subsurface was 11.94% and 29.52%, respectively, the growth of available phosphorus content in surface and subsurface was 27.14% and 54.04%, respectively, and the growth of available potassium content in surface and subsurface was 32.76% and 46.25%, respectively; Compared with ck, fullamount corn stover deep incorporation (CS) significantly increased the content of soil organic carbon (SOC) and soil organic carbon content of humus components, and the contents of SOC, WSS, HA, FA and HM organic carbon in surface and subsurface increased by 30.08% and 32.15%, 137.50% and 157.14%, 63.57% and 63.86%, 4.39% and 6.34%, 24.75% and 25.40%, respectively; Fullamount corn stover deep incorporation (CS) treatment also had the highest degree of humification, and PQ values of the surface and subsurface were 66.35% and 65.18%, respectively; Compared with ck, fullamount corn stover deep incorporation (CS) treatment significantly increased the values of HA and FA tonality coefficient ΔlgK, HA ΔlgK of surface  and subsurface  increased by 7.19% and 12.48%, respectively, and FA ΔlgK increased by 4.19% and 10.18%, respectively. 

2020 Vol. 42 (5): 545-551 [Abstract] ( 16 ) [HTML 1KB] [PDF 1060KB] ( 0 )
552 LIU Shidan, LU Jingkun, HU Ning, WANG Hongbin, ZHAO Lanpo
Effects of Temperature and Moisture on Organic Carbon Mineralization in Black Soil Hot!

The study of the effects of temperature and moisture on mineralization of black soil organic carbon is of great significance to reveal the law and mechanism of organic carbon mineralization in black soil under the background of climate change. Through a 280 d indoor culture experiment, changes of the total organic carbon and light and recombined organic carbon content, composition of aggregates and distribution characteristics of the total organic carbon in different grade aggregates were studied in two black soils with different organic carbon content under different temperature and moisture interactions. The results showed that the total organic carbon and light and recombined organic carbon content of the two black soils decreased with the increase of temperature when water content was 5%-50%; At 10-30 ℃, the total organic carbon and light and heavy organic carbon content of high organic carbon soil decreased to the minimum value when water content of high organic carbon soil was 29% (equivalent to about 70% of field water capacity) and water content of low organic carbon soil was 23% (equivalent to 60%-70% of field water capacity). In the mineralized total organic carbon, the mineralization ratio of light group was higher than that of heavy group, the mineralization rate of the total organic carbon and light and heavy organic carbon in high organic carbon soil was higher than that in low organic carbon soil, and the response of high organic carbon soil to temperature and water change was more sensitive than that of low organic carbon soil. After 280 d of incubation under different temperature and moisture conditions, the macro aggregates > 025 mm tended to transform into < 025 mm micro aggregates. When temperature was 30 ℃, soil water content of high organic carbon and 29% and that of low organic carbon soil was 23%, and the mineralization of organic carbon in large aggregates was the largest. 

2020 Vol. 42 (5): 552-560 [Abstract] ( 20 ) [HTML 1KB] [PDF 1361KB] ( 6 )
561 ZHANG Qun, TANG Xian, ZHOU Haiyan, CAI Zejiang, XU Minggang, WEN Shilin, GAO Qiang
Acidity Characteristics of Paddy Soil Profiles Derived from Different Parent Materials Hot!

To investigate acidity characteristics at different depths of paddy soil derived from different parent materials, a series of soil samples at different depths were collected from 25 sites with 4 parent materials (red sandstone, river alluvium, quaternary red clay and plate shale) to be determined and analyzed. The results indicated, at 0-20 cm soil layers, paddy soil acidity was larger than that of 60-100 cm soil layers, soil exchangeable calcium (Ca2+) and magnesium( Mg2+) derived from contents quaternary red clay parent materials were significantly higher than that of plate shale parent material, and there were no significant difference between red sandstone and river alluvium parent materials. Compared with 0-100 cm soil layers, at 20-80 cm soil layers, paddy soil pH increased significantly by 0.54-1.26, exchangeable hydrogen(H+) content decreased significantly by 0.12-0.15 cmol/kg, exchangeable aluminum(Al3+) content decreased significantly by 1.06-1.38 cmol/kg, exchangeable Ca2+content increased significantly by 0.81-2.22 cmol/kg, exchangeable Mg2+content increased significantly by 0.30-1.08 cmol/kg, there is no significance on different parent materials; At 80-100 cm soil layers, soil pH and exchangeable Ca2+ contents derived from quaternary red clay, red sandstone and river alluvium parent materials were significantly higher than that of plate shale parent material. Soil exchangeable Al3+ content derived from plate shale parent material were significantly higher than that of quaternary red clay parent material, and there were no significant difference between red sandstone and river alluvium. Soil exchangeable Mg2+ content derived from quaternary red clay, red sandstone and plate shale parent materials were significantly higher than that of river alluvium parent material. Paddy soil pH derived from different parent materials significantly positively correlated with exchangeable H+and Al3+, organic matter and total nitrogen, but negatively correlated with exchangeable Ca2+and Mg2+ (P< 0.01). At 80-100 cm soil layers, the acidity of paddy soil was larger at 0-20 cm soil layer, affected by exchangeable H+and Al3+, exchangeable Ca2+and Mg2+, organic matter and total nitrogen, at 80-100 cm soil layers, soil acidity variability was less, affected by parent materials, showing  that acidity of paddy soil derived from red sandstone, river alluvium, and quaternary red clay parent materials was less than that of plate shale.

2020 Vol. 42 (5): 561-568 [Abstract] ( 17 ) [HTML 1KB] [PDF 1461KB] ( 0 )
569 WANG Han, ZHANG Zhongqing, LIU Jinhua, LI Na, LIU Yang, DOU Sen,YANG Jingmin
Improvement Effect of Different Amendments on Soda Saline-alkali Soil Hot!
The effects of four different amendments on the physicochemical properties of salinealkali soil and the growth of maize seedlings were studied by pot experiments. The results showed that, after being treated with the four amendments, pH and watersoluble salt content of the soil were significantly reduced. In contrast, the contents of soil organic matter, available phosphorus, available potassium and alkalihydrolyzable nitrogen increased. The contents of CO32-,HCO3-,Ca2+,Cl-and Na+ in the soil decreased, and the contents of Mg2+ and K+ increased. The SO42- content changed little. After comprehensive analysis, the effect of G3was the best.
2020 Vol. 42 (5): 569-575 [Abstract] ( 21 ) [HTML 1KB] [PDF 1079KB] ( 0 )
576 LU Zhou, HOU Zhiguang
Dissipation and  Residues  of Diflufenican in Rice
The dissipation kinetics and final residues of pesticides in crops are crucial factors to evaluate the safety of pesticide application. In this study, the dissipation and final residues of the herbicide diflufenican in rice were investigated under field conditions in different rice cultivation areas in China using highperformance liquid chromatographytandem mass spectrometry (HPLC-MS/MS). The results showed that mean recoveries of diflufenican from rice plant, rice hull and brown rice were 90.3%-102.7%, 78.0%-96.0% and 92.7%-97.9%, respectively, and the corresponding relative standard deviations (RSD) were 1.0%-4.8%, 4.4%-6.7% and 1.4%-4.6%, respectively. Halflives of diflufenican in rice plant were in the range of 0.6-9.7 d. At harvest time, the residues of diflufenican in rice plant were lower than 0.01 mg/kg, and the values in rice hull and brown rice were less than 0.005 mg/kg when diflufenican was applied once at the dose of active ingredient of 150-225 g/hm2. Based on the results, we suggested that the maximum residue limit (MRL) of diflufenican in brown rice could be established at 0.01 mg/kg in China.
2020 Vol. 42 (5): 576-580 [Abstract] ( 20 ) [HTML 1KB] [PDF 1430KB] ( 0 )
581 LI Wanhong, YUE Xiangpeng, LI Xicheng, WENG Xiuxiu, LI Fadi
Effects of Gallic Acid on Apoptosis of Mice TM3 Leydig Cells

The present study was undertaken to elucidate direct effect of Gallic acid (GA), one of the phenolic compound, on mice TM3 Leydig cell apoptosis, using WST-1, JC-1, DAPI and realtime PCR techniques. The results showed that after adding different concentrations of GA (0, 20, 100, 200 and 400 μmol/L) to the culture system for 24 h, the viability of TM3 cells was downregulated in a dosedependent manner. Compared with control group, the viability of TM3 cells in 20 to 400 μmol/L GA treatment groups was significantly decreased(P<0.05). GA inhibited the expression of cell proliferation related genes Cyclin B1 and PCNA mRNA(P<0.05), on the contrary, it promoted the expression of apoptosis related gene BAX mRNA, which resulted in mitochondrial membrane potential decrease, nuclear condensation and apoptotic body formation. In conclusion, GA had a significant inhibitory effect on the viability of TM3 cells, and thus promoted the apoptosis of TM3 cells.

2020 Vol. 42 (5): 581-586 [Abstract] ( 27 ) [HTML 1KB] [PDF 2109KB] ( 0 )
587 LI Jian, GUO Yingjie, WANG Jingli
Subsoiling Shovel Type Resistance Measurement of Soda SalineAlkali Soil

Aiming at the physical and chemical properties of soda salinealkali soil in Northeast China, depth, speed and shovel type of subsoiling were selected as test factors by using field mechanical dynamic parameter telemeter, and resistance of subsoiling was taken as test index. Data analysis was carried out by using Design Expert software. The results show that the order of the significance of the effects of various factors on the working resistance is subsoiling depth>working speed>subsoiling shovel type. When the depth of subsoiling is 35 cm, the working speed is 0.83 m/s, and the subsoiling shovel type is chisel subsoiling shovel, the working resistance is the smallest.

2020 Vol. 42 (5): 587-590 [Abstract] ( 16 ) [HTML 1KB] [PDF 2359KB] ( 2 )
吉林农业大学学报
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Copyright 2009 Journal of Jilin Agricultural Univerisity
 No.2888 of Xincheng Dajie,Changchun City,Jilin Province(130118)
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