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| Effects of Gallic Acid on Apoptosis of Mice TM3 Leydig Cells |
| LI Wanhong1, YUE Xiangpeng1, LI Xicheng2, WENG Xiuxiu1, LI Fadi1,3** |
| 1. State Key Laboratory of Grassland Agroecosystems， Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs， Engineering Research Center of Grassland Industry, Ministry of Education， College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China; 2. Extending Station for Animal Husbandry and Veterinary of Dachaigou Town in Tianzhu Tibetan Autonomous County, Tianzhu 733201, China; 3. Biotechnology Engineering Laboratory of Gansu Meat Sheep Breeding, Minqin 733300, China |
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Abstract The present study was undertaken to elucidate direct effect of Gallic acid (GA), one of the phenolic compound, on mice TM3 Leydig cell apoptosis, using WST-1, JC-1, DAPI and realtime PCR techniques. The results showed that after adding different concentrations of GA (0, 20, 100, 200 and 400 μmol/L) to the culture system for 24 h, the viability of TM3 cells was downregulated in a dosedependent manner. Compared with control group, the viability of TM3 cells in 20 to 400 μmol/L GA treatment groups was significantly decreased（P＜0.05）. GA inhibited the expression of cell proliferation related genes Cyclin B1 and PCNA mRNA（P＜0.05）, on the contrary, it promoted the expression of apoptosis related gene BAX mRNA, which resulted in mitochondrial membrane potential decrease, nuclear condensation and apoptotic body formation. In conclusion, GA had a significant inhibitory effect on the viability of TM3 cells, and thus promoted the apoptosis of TM3 cells.
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Received: 09 April 2018
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