The dissipation kinetics and final residues of pesticides in crops are crucial factors to evaluate the safety of pesticide application. In this study, the dissipation and final residues of the herbicide diflufenican in rice were investigated under field conditions in different rice cultivation areas in China using highperformance liquid chromatographytandem mass spectrometry (HPLC-MS/MS). The results showed that mean recoveries of diflufenican from rice plant, rice hull and brown rice were 90.3%-102.7%, 78.0%-96.0% and 92.7%-97.9%, respectively, and the corresponding relative standard deviations (RSD) were 1.0%-4.8%, 4.4%-6.7% and 1.4%-4.6%, respectively. Halflives of diflufenican in rice plant were in the range of 0.6-9.7 d. At harvest time, the residues of diflufenican in rice plant were lower than 0.01 mg/kg, and the values in rice hull and brown rice were less than 0.005 mg/kg when diflufenican was applied once at the dose of active ingredient of 150-225 g/hm2. Based on the results, we suggested that the maximum residue limit (MRL) of diflufenican in brown rice could be established at 0.01 mg/kg in China.