The study of the effects of temperature and moisture on mineralization of black soil organic carbon is of great significance to reveal the law and mechanism of organic carbon mineralization in black soil under the background of climate change. Through a 280 d indoor culture experiment, changes of the total organic carbon and light and recombined organic carbon content, composition of aggregates and distribution characteristics of the total organic carbon in different grade aggregates were studied in two black soils with different organic carbon content under different temperature and moisture interactions. The results showed that the total organic carbon and light and recombined organic carbon content of the two black soils decreased with the increase of temperature when water content was 5%-50%; At 10-30 ℃, the total organic carbon and light and heavy organic carbon content of high organic carbon soil decreased to the minimum value when water content of high organic carbon soil was 29% (equivalent to about 70% of field water capacity) and water content of low organic carbon soil was 23% (equivalent to 60%-70% of field water capacity). In the mineralized total organic carbon, the mineralization ratio of light group was higher than that of heavy group, the mineralization rate of the total organic carbon and light and heavy organic carbon in high organic carbon soil was higher than that in low organic carbon soil, and the response of high organic carbon soil to temperature and water change was more sensitive than that of low organic carbon soil. After 280 d of incubation under different temperature and moisture conditions, the macro aggregates > 025 mm tended to transform into < 025 mm micro aggregates. When temperature was 30 ℃, soil water content of high organic carbon and 29% and that of low organic carbon soil was 23%, and the mineralization of organic carbon in large aggregates was the largest. 