To clarify the migration activity of exogenous nitrogen on acidified black soil in protected area, batch equilibrium adsorption experiment was used to explore the adsorption characteristics of nitrogen under different influencing factors. The results showed that the adsorption process of nitrogen in acidified black soil in protected area included rapid, decelerated and equilibrium stages, quasisecondorder kinetics combined with Elovich equation could describe the adsorption kinetics of nitrogen in acidified soil in protected areas; With the increase of nitrogen concentration, the adsorption capacity of the tested soil to nitrogen increased first and then tended to balance, and the adsorption capacity to nitrogen increased with the increase of ambient temperature. When the content of organic matter in soil decreased from 33.95 g/kg to 19.9 g/kg, the saturated adsorption capacity decreased by 17.4%. The addition of exogenous ions in the background solution inhibited the adsorption of nitrogen by soil. The alkalization of the tested soil could promote nitrogen fixation and reduce nitrogen loss. The results could reveal the environmental behavior of nitrogen in acid soil and provide theoretical basis for reducing groundwater pollution.