In order to obtain the cabbage plant resistant to turnip mosaic virus disease and bolting, the construction and genetic transformation of bivalent antisense plant expression vectors were studied. Using TuMV-Nib and LFY genes, an antisense bivalent plant expression vector was constructed and transformed into Agrobacterium LBA4404. Based on the engineering strain, Agrobacteriummediated vacuum infiltration method was used to transform 4 Chinese cabbage inbred lines, and 16 transgenic plants identified by PCR and TuMV infection were obtained. Seven strains were identified as resistant to TuMV virus and bolting in the field. It provides germplasm materials for transgenic Chinese cabbage resistant to antiviral diseases and bolting.