The cultures of white rot fungus Lenzites gibbosa were treated with anthraquinone dye alizarin red for 0 h (ck), 3 h (QSH1), 7 h (QSH2), and 10 h (QSH3), respectively, and 4 transcriptomes were constructed so as to provide support for the study on the role of cytochrome P450 (CYP450) genes in the decolorization and degradation of alizarin red by white rot fungi. The highthroughput sequencing technology was used to perform transcriptome sequencing on the hyphal samples under the treatment of alizarin red. The cytochrome P450 genes were screened out using functional annotation. Results showed that a total of 26.17 Gb of valid data were obtained from the four transcriptomes, and the mapped ratios between the reads and reference genome of each sample ranged from 71.23% to 73.66%. Ninetysix CYP450 genes were gained through searching all genes by functional annotation. Differential expression screening was performed for all CYP450 genes by comparing ck with QSH1, ck with QSH2, and ck with QSH3. Three of the genes, gene_24255, gene_18992, and gene_7490, were found in all three comparative groups. The expression levels of the three genes at 0-10 h were compared with the decolorization rate of alizarin red, and the expression quantities of the three genes were positively correlated with decoloring rate. Through the analysis of the KEGG pathway, gene_25488 was annotated into the pathway of aromatic degradation.The results indicated that CYP450 genes may be involved in the decolorization of alizarin red dye by L.gibbosa. This study, at the transcriptional level, preliminarily analyzed the CYP450 genes and their products of L. gibbosa, which may be involved in the related metabolic pathways and biological processes of degradation and decolorization of alizarin red. The results obtained have important reference value for the subsequent research on the degradation mechanism of alizarin red.